102185-49-9 , BCIP dipotassium, BCIP.2K; X-Phosphate dipotassium salt; 5-Bromo-4-chloro-3-indolyl phosphate dipotassium salt

102185-49-9 , BCIP dipotassium,
BCIP.2K; X-Phosphate dipotassium salt; 5-Bromo-4-chloro-3-indolyl phosphate dipotassium salt
C8H4BrClK2NO4P / 402.65

5-Bromo-4-chloro-3-indolyl phosphate dipotassium salt

BCIP dipotassium,BCIP.2K; X-Phosphate dipotassium salt;

BCIP (5-bromo-4-chloro-3-indolyl phosphate) dipotassium is a chromogenic substrate commonly used for the detection of the enzymatic activity of alkaline phosphatase. Upon dephosphorylation by alkaline phosphatase, BCIP produces a blue precipitate, which can be easily visualized. This substrate has various uses, including the detection of gene expression in molecular biology, the identification of alkaline phosphatase activity in clinical pathology, and the detection of protein-protein interactions in biochemistry. Its long-term stability in solution makes it a common choice for many applications.

CAS Number	102185-49-9
Product Name	Potassium 5-bromo-4-chloro-1H-indol-3-yl phosphate
IUPAC Name	dipotassium;(5-bromo-4-chloro-1H-indol-3-yl) phosphate
Molecular Formula	C8H4BrClK2NO4P
Molecular Weight	402.65 g/mol
InChI	InChI=1S/C8H6BrClNO4P.2K/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
InChI Key	BYVJPKSAOAYTGG-UHFFFAOYSA-L
SMILES	C1=CC(=C(C2=C1NC=C2OP(=O)([O-])[O-])Cl)Br.[K+].[K+]
Canonical SMILES	C1=CC(=C(C2=C1NC=C2OP(=O)([O-])[O-])Cl)Br.[K+].[K+]