4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2→3)-O-b-D-galactopyranosyl-(1→3)-2-acetamido-2-deoxy-a-D-galactopyranoside

Neu5Aca(2-3)Galb(1-3)GlcNAc-a-pNP; a-pNP 3'-Sialylgalacto-N-bioside

4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2,3)-O-b-D-galactopyranosyl-(1,3)-2-acetamido-2-deoxy-a-D-galactopyran oxide is a fluorogenic substrate that is used in the detection of glycosidases. It has been shown to be an excellent ligand for the enzyme beta galactosidase, which cleaves the glycan substrate 4NPOBGPA into 4NP and O-(N acetyl) neuraminic acid. 4NPOBGPA can be used as a chromogenic substrate for detecting beta galactosidase activity and can also be conjugated to other fluorescent molecules.